Anti-EIF5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55010

Core Product Specifications and Parameters

Biological Background: Eukaryotic Translation Initiation Factor 5 (eIF5) Function and Localization

• Eukaryotic translation initiation factor 5 (eIF5) is encoded by the EIF5 gene and is a key component of the 43S pre-initiation complex (43S PIC) that scans the mRNA 5' untranslated region to locate the start codon. Related references: PMID:11166181, PMID:22813744, PMID:24319994
• Within the 43S PIC, eIF5 acts as a GTPase-activating protein (GAP) that stimulates GTP hydrolysis by the eIF2 gamma subunit (EIF2S3). Related references: PMID:11166181
• During scanning, the N-terminal domain (NTD) of eIF5 interacts with EIF1A, while its C-terminal domain (CTD) binds EIF1, helping maintain EIF1 in the open PIC conformation. Related references: PMID:22813744, PMID:24319994
• Upon start codon recognition, eIF5 NTD triggers eIF2γ GTP hydrolysis, inducing a closed PIC state that increases CTD affinity for EIF2β, releases EIF1, and stabilizes the closed complex. Related references: PMID:11166181, PMID:22813744
• eIF5 localizes predominantly to the cytoplasm where translation initiation occurs.
• The protein has a theoretical molecular weight of approximately 49 kDa and is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• For western blotting (WB), consider using a total protein loading control and optimizing primary antibody dilution based on the approximately 49 kDa target band.
• When performing immunofluorescence (IF/ICC), include appropriate permeabilization and blocking steps; validate antibody specificity in the relevant species (human, mouse, rat).
• For ELISA, use the recombinant EIF5 immunogen (amino acids 1-235) as a positive control to generate a standard curve.
• The antibody is raised against full-length human EIF5 N-terminal region (aa 1-235); confirm reactivity in your sample system and consider cross-reactivity with other eIF5 family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers seamless access to validated IVD raw materials, including this polyclonal antibody targeting the translation initiation factor EIF5. Beyond individual reagents, we provide optimized antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare targets across the protein biosynthesis pathway. Partner with CamelBio to streamline your assay development from concept to clinic with reliable, scalable supply.