Anti-EIF4G2/p97 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P78344

Core Product Specifications and Parameters

Biological Background: EIF4G2/p97 Function and Localization

• EIF4G2 (also known as p97, DAP5, AAG1, NAT1) is a translation initiation factor that functions as a scaffold protein in the eIF4F complex.
• It plays a critical role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis, and viral infection.
• EIF4G2 is a substrate for caspase cleavage during apoptosis, generating fragments that may modulate translation.
• The protein is ubiquitously expressed, with highest levels in skeletal muscle and heart, and is also present in fetal tissues.
• It is subject to multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which may regulate its activity.
• Keywords from UniProt indicate its involvement in protein biosynthesis, translation regulation, and it may act as a repressor of translation in certain contexts.
• The molecular weight is approximately 102 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region of EIF4G2; consider that the antibody may detect both full-length and cleaved fragments depending on experimental conditions.
• For Western blot, a band around 102 kDa is expected; include positive control lysates from tissues with high expression, such as skeletal muscle or heart.
• In immunofluorescence, EIF4G2 is mainly cytoplasmic, but nuclear localization may occur in specific contexts.
• For immunoprecipitation, EIF4G2 interacts with other initiation factors; include appropriate controls to confirm specificity.
• Validate the antibody in your relevant sample system, as expression and modification states can vary by cell type and treatment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a one-stop procurement platform for IVD raw materials, including the EIF4G2/p97 polyclonal antibody for apoptosis and translation studies. Our comprehensive supply chain covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.