Anti-EIF4G2/p97 Polyclonal Antibody for WB, ELISA - P78344

Core Product Specifications and Parameters

Biological Background: EIF4G2/p97 Function and Localization

• EIF4G2 (also known as DAP5, p97) is a member of the eIF4G family of translation initiation factors, involved in regulating protein synthesis.
• It functions as a scaffold protein that mediates cap-independent translation initiation via internal ribosome entry sites (IRES), especially under cellular stress conditions.
• Plays a critical role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis, and viral infection.
• Cleavage by caspases and viral proteases generates fragments that may modulate its activity, contributing to translational reprogramming.
• Ubiquitously expressed, with highest levels in skeletal muscle and heart; also present in fetal tissues, reflecting its broad regulatory roles.
• Subject to post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which likely regulate its activity and interactions.
• Contains a structured domain; 3D structure has been partially resolved, aiding understanding of its molecular interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 750-850, a region that may be important for function; consider assessing binding to native and denatured protein in your specific assay.
• For Western blotting, a major band at ~102 kDa is expected; additional bands may appear in apoptotic or virally infected cells due to known cleavage events.
• Cross-reactivity with mouse and rat is indicated; however, confirm sequence homology in the target region to ensure recognition.
• In ELISA, consider using recombinant protein or peptide-coated plates for capture, and validate with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing bridges the gap from concept to clinic, ensuring consistent supply for your translation regulation research and assay development. Contact us to discuss custom antibody sourcing and IVD raw material solutions for EIF4G2/p97 targets.