Anti-EIF4G1 Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q04637

Core Product Specifications and Parameters

Biological Background: EIF4G1 Function and Localization

• EIF4G1 is a component of the eIF4F complex that recognizes the mRNA cap, unwinds 5'-terminal secondary structure, and recruits mRNA to the ribosome. Related references: PMID:29987188
• It forms two mutually exclusive subcomplexes: one with EIF1 and another with EIF4E, each playing distinct roles in translation initiation. Related references: PMID:29987188
• In complex with EIF1, EIF4G1 promotes leaky scanning to avoid cap-proximal start codons. Related references: PMID:29987188
• With EIF4E, it facilitates start codon location via a TISU element without scanning. Related references: PMID:29987188
• As part of eIF4F, EIF4G1 is required for endoplasmic reticulum stress-induced ATF4 mRNA translation. Related references: PMID:29062139
• The protein localizes predominantly in the cytoplasm, but also in the nucleus and stress granules, consistent with its roles in translation and stress responses.
• EIF4G1 undergoes alternative initiation and splicing, yielding multiple isoforms; it is subject to acetylation, methylation, and phosphorylation.
• Mutations in EIF4G1 are linked to Parkinson disease and neurodegeneration, underscoring its critical cellular functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 400–500 of human EIF4G1 (NP_886553.3); the antibody may recognize isoforms or fragments containing this epitope.
• For Western blotting, the predicted molecular weight is ~175 kDa; consider using a 6–8% SDS-PAGE gel and extended transfer times for efficient blotting.
• Validate reactivity in the appropriate human cell or tissue lysates; cross-reactivity with other species is not reported.
• For immunohistochemistry on paraffin-embedded sections, optimize antigen retrieval and antibody dilution.
• The antibody is reported for immunoprecipitation and ELISA; empirical titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-EIF4G1 polyclonal antibody supports research into translation initiation, stress responses, and neurodegenerative disorders. For specialized needs or rare target sourcing, CamelBio streamlines your workflow with validated reagents and customized support.