Anti-EIF4G1 Polyclonal Antibody for WB and ELISA - Q04637

Core Product Specifications and Parameters

Biological Background: EIF4G1 Function and Localization

• Component of the eIF4F complex, responsible for mRNA cap recognition, ATP-dependent unwinding of 5'-UTR secondary structures, and ribosome recruitment. Related references: PMID:29987188
• Exists in two mutually exclusive subcomplexes with EIF1 (promoting leaky scanning) or EIF4E (antagonizing scanning for start codon selection). Related references: PMID:29987188
• Mediates endoplasmic reticulum stress-induced translation of ATF4, linking translational control to cellular stress responses. Related references: PMID:29062139
• Localizes to the cytoplasm, nucleus, and stress granules, reflecting its dynamic role in translation regulation under normal and stress conditions.
• Undergoes extensive post-translational modifications, including phosphorylation and acetylation; mutations are associated with Parkinson disease (PARK18).
• Alternatively spliced isoform production and interactions with viral proteins highlight its versatility in diverse biological contexts (host-virus interaction).

Experimental Guidance and Technical Tips

• For western blot (WB), expect a major band at ~175 kDa; use a suitable molecular weight marker and validate with human-derived cell lysates.
• Optimize primary antibody concentration by testing a dilution series (e.g., 1:500–1:2000) in the relevant sample system.
• The immunogen (aa 550–650) lies within a conserved central region; potential cross-reactivity with other EIF4G family members should be evaluated when analyzing non-human samples.
• For ELISA, consider peptide-based assay design; thorough optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies for translation initiation research. With expertise in rare target sourcing and bulk ancillary reagent supply, we support the development of robust immunoassays targeting EIF4G1 and related pathways. Contact us for custom polyclonal antibody production and comprehensive technical consultation.