Anti-eIF4EBP1 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• Eukaryotic translation initiation factor 4E-binding protein 1 (eIF4EBP1), also known as PHAS-I, is a key repressor of cap-dependent translation initiation. It functions by binding to the eIF4E subunit and preventing its assembly into the eIF4F complex.
• Hypophosphorylated eIF4EBP1 competes with the scaffold protein eIF4G for a shared binding site on eIF4E, thereby inhibiting translation. Hyperphosphorylation in response to growth factors, hormones, or nutrients dissociates eIF4EBP1 from eIF4E, allowing eIF4F complex formation and translation initiation.
• The activity of eIF4EBP1 is primarily regulated through the mTORC1 and MAP kinase signaling pathways, integrating extracellular and intracellular cues to control protein synthesis.
• eIF4EBP1 is predominantly localized in the cytoplasm but can also be detected in the nucleus, consistent with its roles in translation regulation and possibly export of eIF4E.
• Post-translational modifications, particularly phosphorylation at multiple sites, acetylation, and ubiquitination, dynamically modulate its affinity for eIF4E and its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (amino acids 1-118) of human eIF4EBP1, which covers a region critical for eIF4E binding and phosphorylation-dependent regulation. This suggests the antibody may recognize both phosphorylated and unphosphorylated forms, but validation in the relevant sample system is advised.
• For IHC-P applications, consider antigen retrieval conditions suitable for nuclear and cytoplasmic antigens, and include appropriate tissue controls (e.g., tissues with known high/low eIF4EBP1 expression). Cross-reactivity with mouse and rat indicates potential utility in these species.
• For ELISA, use recombinant eIF4EBP1 protein or cell lysates as coating antigens, and test antibody dilutions in a checkerboard format. Since the antibody is a polyclonal rabbit pAb, signal may vary between batches.
• As a polyclonal antibody, it offers broad epitope recognition, which can be advantageous for detecting multiple isoforms or modifications, but it may also require careful blocking and secondary antibody selection to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents. This anti-eIF4EBP1 polyclonal antibody supports research into translation regulation, mTOR signaling, and related therapeutic areas. Whether you need ready-to-use immunoreagents or assistance sourcing rare-target raw materials, CamelBio’s one-stop platform bridges the gap between concept and clinic, ensuring your supply chain for reliable, high-quality components.