Anti-eIF4EBP1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• eIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1), also known as PHAS-I, is a repressor of translation initiation.
• It regulates EIF4E activity by competitive binding; the hypophosphorylated form of eIF4EBP1 binds to EIF4E, preventing the assembly of the eIF4F complex and inhibiting cap-dependent translation.
• Hyperphosphorylation in response to growth factors, hormones, or nutrients (via MAP kinase and mTORC1 pathways) promotes dissociation from EIF4E, enabling eIF4F formation and translation initiation.
• eIF4EBP1 localizes to both the cytoplasm and nucleus, consistent with its dynamic role in translation regulation.
• Post-translational modifications including phosphorylation, acetylation, and ubiquitination modulate its activity and stability.
• As a critical downstream effector of the mTOR signaling pathway, eIF4EBP1 links nutrient-sensing and growth signals to protein synthesis.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–118, a central region conserved across species; it may detect both phosphorylated and unphosphorylated forms, but phosphorylation-dependent epitope masking should be considered in experimental design.
• For applications requiring quantitative phospho-specific detection, consider pairing this antibody with a phospho‑eIF4EBP1-specific antibody.
• Due to its small molecular weight (~13 kDa), ensure optimal gel percentage (12–15% SDS-PAGE) and transfer conditions in western blotting.
• Validation in the relevant sample system and treatment conditions is recommended, as eIF4EBP1 phosphorylation status varies with cell state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-eIF4EBP1 product. From bulk ancillary reagents to rare target raw-material sourcing, our platform bridges your entire development workflow. With expertise spanning from concept to clinic, CamelBio is your reliable partner for translation research and mTOR pathway studies.