Anti-eIF4EBP1 (4E-BP1) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• Eukaryotic translation initiation factor 4E-binding protein 1 (eIF4EBP1), also known as PHAS-I, is a key repressor of cap-dependent translation initiation.
• In its hypophosphorylated state, eIF4EBP1 competes with EIF4G1/EIF4G3 for binding to EIF4E, preventing assembly of the eIF4F complex and inhibiting translation.
• Phosphorylation by mTORC1 and MAP kinase pathways in response to growth factors, hormones, and nutrients converts eIF4EBP1 to a hyperphosphorylated form that releases EIF4E, allowing translation initiation.
• eIF4EBP1 shuttles between the cytoplasm and nucleus, and its function is essential for cellular growth, proliferation, and survival signaling.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which fine-tune its activity and stability.
• As a central node in translation control, eIF4EBP1 is implicated in cancer, metabolic disorders, and neurodegenerative diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved N-terminal region (aa 20-118), which may recognize multiple phospho-isoforms; consider verifying specificity in your model system.
• For Western blot, the predicted molecular weight is 13 kDa; use low-molecular-weight markers and optimize transfer conditions to detect this small protein.
• In immunohistochemistry and immunofluorescence, signal may be diffuse due to both cytoplasmic and nuclear localization; include appropriate subcellular markers.
• When studying mTOR pathway activation, compare band shifts or signal intensity changes upon treatment with inhibitors such as rapamycin.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For translational and oncology research, our eIF4EBP1 rabbit monoclonal antibody is just one example of high-quality reagents we supply. We offer validated antibody pairs, bulk ancillary reagents, and customized procurement to accelerate your development.