Anti-eIF4E Polyclonal Antibody for WB, IF/ICC, ELISA - P06730

Core Product Specifications and Parameters

Biological Background: eIF4E Function and Localization

• Eukaryotic translation initiation factor 4E (eIF4E) is also known as eIF-4F 25 kDa subunit or mRNA cap-binding protein, encoded by the EIF4E gene. It is a central regulator of protein synthesis.
• Recognizes and binds the 7-methylguanosine (m7G) cap at the 5' end of mRNAs as a core component of the eIF4F complex, facilitating unwinding of mRNA secondary structures and ribosome recruitment for translation initiation. Related references: PMID:16271312 PMID:22578813
• Mediates nuclear export of a subset of mRNAs, including cyclin D1 (CCND1), NOS2, and MDM2, through recognition of the 4ESE element and assembly of a transport complex involving LRPPRC and XPO1. Related references: PMID:23471078 PMID:22684010
• Functions in translational repression as part of the CYFIP1-EIF4E-FMR1 complex and participates in the storage of translationally inactive mRNAs in cytoplasmic processing bodies (P-bodies). Related references: PMID:24335285
• Plays non-canonical roles in the nucleus, promoting cap methylation, pre-mRNA splicing, and 3'-end processing, partly by facilitating the export and translation of RNAs encoding capping and cleavage factors. Related references: PMID:11606200 PMID:22578813 PMID:22684010
• Subject to post-translational modifications including phosphorylation and acetylation, which modulate its activity; has been implicated in spermatogenesis and anti-apoptotic signaling through regulation of specific mRNA transport and translation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human eIF4E protein (amino acids 1–217). This antibody is expected to detect endogenous eIF4E at approximately 25 kDa in human samples.
• For Western blot (WB), consider using whole-cell lysates from HeLa, HEK293, or Jurkat cells as positive controls. A dilution range of 1:500–1:2000 is common for polyclonal antibodies, but optimization is recommended.
• In immunofluorescence/immunocytochemistry (IF/ICC), eIF4E localizes mainly to the cytoplasm, with nuclear and P-body staining possible depending on cell type and conditions; validate staining patterns in your specific system.
• For ELISA applications, this antibody can be used as a capture or detection reagent; titrate to determine the optimal working concentration for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and customized solutions to accelerate your diagnostic development. This anti-eIF4E polyclonal antibody supports research into translational control, cancer biology, and neurological disorders. Contact us to explore our comprehensive portfolio of translation-related reagents and technical support tailored to your needs.