Anti-EIF4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P23588

Core Product Specifications and Parameters

Biological Background: EIF4B Function and Localization

• EIF4B (Eukaryotic translation initiation factor 4B) is an RNA-binding protein essential for the recruitment of mRNA to ribosomes during the initiation phase of protein synthesis.
• It functions cooperatively with the EIF4-F cap-binding complex and the DEAD-box RNA helicase EIF4-A, facilitating ATP-dependent unwinding of mRNA secondary structures near the 5' cap.
• By stimulating the ATPase and helicase activities of EIF4-A/EIF4-F, EIF4B enhances the scanning of the 5' untranslated region for the start codon.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which regulate its activity and interactions.
• Alternative splicing generates distinct isoforms, potentially expanding functional diversity.
• EIF4B's RNA-binding domains and interaction with translation machinery place it at a critical node of translational control, relevant to studies on cancer, cellular stress, and viral infections.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant fragment spanning amino acids 352–611 (C‑terminal region) of human EIF4B, which is highly conserved between human and mouse.
• It has been qualified for western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), immunoprecipitation (IP), and ELISA. For WB, a band near 69 kDa is expected.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays, and optimal working dilutions must be determined experimentally in the relevant sample matrix.
• For IP, pre‑clearing lysates and using appropriate antibody‑isotype controls are recommended to reduce nonspecific binding.
• In IF/ICC, permeabilization and fixation conditions should be optimized, and counterstaining with a nuclear marker may help assess localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutes with high-quality reagents like this anti-EIF4B polyclonal antibody. Our portfolio spans validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for even the most challenging targets. No matter your translation‑focused project or assay development needs, CamelBio streamlines procurement from concept to clinic.