Anti-EIF4B Polyclonal Antibody for WB, IF/ICC, ELISA - P23588

Core Product Specifications and Parameters

Biological Background: EIF4B Function and Localization

• EIF4B (Eukaryotic translation initiation factor 4B) is a 69.2 kDa protein that functions as a translation initiation factor, essential for the binding of mRNA to ribosomes.
• It operates in close association with the eIF4F complex and eIF4A, binding near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP.
• EIF4B promotes the ATPase activity and ATP-dependent RNA unwinding activity of both eIF4A and eIF4F, thereby facilitating ribosomal scanning.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which likely regulate its activity.
• Alternative splicing generates several isoforms of EIF4B, potentially contributing to functional diversity.
• EIF4B contains RNA-binding domains and is classified under keywords such as Initiation factor, Protein biosynthesis, and RNA-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 352-611) of human EIF4B, a segment that may be conserved across human, mouse, and rat, aligning with the reported cross-reactivity.
• For Western blot, a band near 69 kDa is expected; consider using lysates from HeLa or HEK293 cells as positive controls and optimize antibody dilution for your specific system.
• In immunofluorescence/ICC applications, validate the staining pattern in EIF4B-expressing cells and include subcellular compartment markers as references.
• For ELISA, perform titration experiments to determine the optimal antibody and antigen concentrations; recombinant EIF4B protein can serve as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-EIF4B polyclonal antibody enables translation initiation studies and assay development. To meet larger volume demands, obtain matched antibody pairs, or source rare-target raw materials, reach out to CamelBio for streamlined procurement and dedicated support.