Anti-eIF4A3 Rabbit Polyclonal Antibody for WB, ELISA - P38919

Core Product Specifications and Parameters

Biological Background: eIF4A3 Function and Localization

• eIF4A3 is an ATP-dependent RNA helicase belonging to the DEAD-box family, essential for ATP-dependent unwinding of RNA secondary structures. Related references: PMID:16170325
• It is a core component of the exon junction complex (EJC), deposited 20–24 nucleotides upstream of exon–exon junctions during pre-mRNA splicing. The EJC influences mRNA export, localization, translation, and nonsense-mediated decay. Related references: PMID:16170325, PMID:16209946, PMID:16314458
• eIF4A3 participates directly in the spliceosome and pre-mRNA splicing machinery. Related references: PMID:11991638, PMID:22961380, PMID:28076346
• It modulates alternative splicing of BCL2L1/Bcl-X, inhibiting the production of pro-apoptotic Bcl-X(S) isoforms, thus affecting apoptosis. Related references: PMID:22203037
• The protein is required for normal craniofacial development. Related references: PMID:24360810
• Subcellularly, eIF4A3 localizes mainly to the nucleus and nuclear speckles, but can shuttle to the cytoplasm as part of the EJC on mature mRNAs.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination (isopeptide bond formation), as indicated by UniProt keywords. Alternative names include DDX48, NMP265, eIF4AIII, and KIAA0111; the protein is ubiquitously expressed.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 210 amino acids of human eIF4A3; antibody reactivity is expected to detect endogenous full-length protein in human, mouse, and rat samples.
• For Western blotting, the predicted molecular weight is approximately 47 kDa; consider using nuclear extracts or whole-cell lysates from proliferating cells, as eIF4A3 is predominantly nuclear and associates with spliceosomes.
• In ELISA applications, the antibody can be used with recombinant eIF4A3 protein or peptide coating; validate assay conditions with appropriate positive and negative controls.
• Verify antibody specificity in your experimental system by assessing knockout or knockdown controls where available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies, recombinant proteins, and specialized reagents for mRNA processing and translational control research. With deep expertise in splicing and EJC biology, we support diagnostic developers and researchers with tailored sourcing solutions—from off-the-shelf antibodies to custom production and bulk supply. Partner with us to streamline your workflow from target discovery to clinical application.