Anti-eIF4A3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P38919

Core Product Specifications and Parameters

Biological Background: eIF4A3 Function and Localization

• Encoded by the EIF4A3 gene, the protein is also known as ATP-dependent RNA helicase DDX48, nuclear matrix protein 265 (NMP265), and eukaryotic initiation factor 4A-III.
• It is an integral component of the spliceosome, essential for pre-mRNA splicing. Related references: PMID:11991638, PMID:22961380, PMID:28076346
• It acts as an ATP-dependent RNA helicase, facilitating ATP-dependent unwinding of RNA secondary structures during pre-mRNA splicing and other processes. Related references: PMID:16170325
• It is a core component of the exon junction complex (EJC), deposited on mRNA at splice junctions to mark exon-exon boundaries and influence post-splicing processes such as nuclear export, subcellular localization, translation efficiency, and nonsense-mediated mRNA decay (NMD). Related references: PMID:16170325, PMID:16209946, PMID:16314458
• The helicase activity is stimulated by CASC3 and inhibited by the MAGOH-RBM8A heterodimer, which stabilizes the EJC on spliced mRNA in an ATP-bound conformation.
• It modulates alternative splicing of apoptotic genes such as BCL2L1, inhibiting the production of the proapoptotic Bcl-X(S) isoform. Related references: PMID:22203037
• It is involved in craniofacial development. Related references: PMID:24360810
• Subcellularly, it localizes to the nucleus, nucleoplasm speckles, and cytoplasm. It is ubiquitously expressed and subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• Immunogen spans amino acids 34–125 of human eIF4A3; thus, the antibody likely recognizes a conserved N-terminal epitope. Consider verifying specificity with recombinant protein or lysates from eIF4A3-overexpressing cells.
• For Western blot, the predicted molecular weight is ~47 kDa. Use whole-cell lysates from human (HeLa, HEK293), mouse, or rat cell lines as positive controls. Validate in your specific sample system.
• In immunofluorescence/ICC, anticipate nuclear (with speckled pattern) and possibly cytoplasmic staining. Co-localization studies with spliceosome or EJC markers may confirm target specificity.
• For ELISA, the antibody can be used with the immunogen or purified eIF4A3 protein. Establish optimal coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibodies, recombinant proteins, and ancillary reagents from concept to clinic. This eIF4A3 antibody supports research into pre-mRNA splicing and exon junction complex biology, and CamelBio can source complementary raw materials—such as other EJC component antibodies, recombinant eIF4A3 protein, or bulk ancillary reagents—to streamline your workflow. Whether you need rare-target reagents or optimized assay components, our one-stop model simplifies procurement and accelerates development.