Anti-EIF4A2 Polyclonal Antibody for WB, ELISA - Q14240

Core Product Specifications and Parameters

Biological Background: EIF4A2 (eIF4A-II) Function and Localization

• EIF4A2 encodes eukaryotic initiation factor 4A-II (eIF4A-II), a ~46 kDa ATP-dependent RNA helicase belonging to the DEAD-box protein family.
• As a subunit of the eIF4F complex, it functions in cap-dependent translation initiation by unwinding secondary structures in the 5'-UTR of mRNAs.
• This activity enables efficient binding of the 40S ribosomal subunit and scanning for the initiator AUG codon.
• Expression is ubiquitous, with predominant cytoplasmic localization where it exerts its role in protein synthesis.
• The protein contains conserved motifs for ATP binding, RNA binding, and helicase activity, and is subject to phosphorylation and alternative splicing.
• Mutations in EIF4A2 are associated with intellectual disability, and the protein interacts with viral components, highlighting its role in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-270) encompassing part of the helicase core, ensuring recognition of full-length EIF4A2; cross-reactivity with mouse and rat is reported.
• In WB, expect a band at approximately 46 kDa; validate antibody specificity using appropriate positive and negative controls (e.g., knockdown or knockout samples).
• For ELISA applications, purify antigen and optimize coating concentration and detection conditions to achieve linearity.
• Given the conserved nature of eIF4A family members, double-check specificity by sequence alignment and testing on relevant paralogs if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable access to validated antibodies, recombinant proteins, and ancillary reagents. For studies on translation initiation and RNA metabolism, we offer this anti-EIF4A2 polyclonal antibody along with bulk ordering and customized IVD raw material solutions. Our integrated supply chain ensures timely delivery from R&D to commercial scale.