Anti-EIF4A1/EIF4A2/EIF4A3 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P60842/Q14240/P38919

Core Product Specifications and Parameters

Biological Background: EIF4A1/EIF4A2/EIF4A3 Function and Localization

• EIF4A1 (also known as DDX2A or eIF4A-I) is an ATP-dependent RNA helicase that serves as a critical subunit of the eIF4F translation initiation complex. Related references: PMID:20156963
• It unwinds secondary structures in the 5' untranslated region (5'-UTR) of mRNAs, facilitating ribosome binding and scanning for the initiator codon, thereby promoting efficient protein synthesis and cell proliferation.
• EIF4A1 belongs to the DEAD-box RNA helicase family, which includes two closely related isoforms: EIF4A2 (DDX2B) and EIF4A3 (DDX48). The antibody targets a conserved N-terminal region shared among these three paralogs.
• Subcellularly, EIF4A1 is primarily cytoplasmic, with enrichment in the perinuclear region and stress granules; it also localizes to the cell membrane under certain conditions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bonds), which may regulate its activity, localization, or stability.
• Key biological keywords associated with EIF4A1 include host-virus interaction, RNA-binding, nucleotide-binding, and helicase activity, reflecting its multifunctional role in translation and beyond.

Experimental Guidance and Technical Tips

• The immunogen is a conserved N-terminal peptide, suggesting the antibody may recognize all three EIF4A family proteins across human, mouse, and rat. Consider verifying specificity for each isoform in your experimental system using knockdown controls.
• For WB, a band of approximately 46 kDa is expected. Use appropriate positive controls and consider molecular weight markers to confirm.
• For IHC-P and IF/ICC, EIF4A1 is mainly cytoplasmic with stress granule localization; permeabilization and antigen retrieval steps may be required. Validate staining patterns with known positive and negative tissues/cells.
• For IP, the antibody is validated for immunoprecipitation; ensure the lysis buffer is compatible and use appropriate IgG controls.
• For ELISA, test linearity and sensitivity with your target antigen; the antibody may be used as a capture or detection reagent.

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