Anti-eIF4A1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P60842

Core Product Specifications and Parameters

Biological Background: eIF4A1 Function and Localization

• Eukaryotic initiation factor 4A-I (eIF4A1) is an ATP-dependent RNA helicase that serves as a subunit of the eIF4F complex, playing a central role in cap-dependent translation initiation.
  Related references: PMID:20156963
• eIF4A1 unwinds RNA secondary structures within the 5'-UTR of mRNAs, facilitating ribosomal binding and scanning for the start codon—a process that promotes cell proliferation and growth.
  Related references: PMID:20156963
• Alternative names include DDX2A, EIF4A, eIF-4A, eIF4A-I, and eIF-4A-I, reflecting its classification as a DEAD box RNA helicase.
• The protein is predominantly found in the cytoplasm, including the perinuclear region, and can also localize to the cell membrane and stress granules.
• eIF4A1 undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination (Ubl conjugation), which likely regulate its function and interactions.
• It participates in host-virus interactions, underscoring its role in cellular defense mechanisms.
• As a key initiation factor for protein biosynthesis, eIF4A1 has been identified in numerous proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1–100). This design often yields high specificity; however, cross-reactivity with other eIF4A isoforms or DEAD box family members should be verified if needed.
• For Western blotting, the expected molecular weight is approximately 46 kDa. Use appropriate positive control lysates (e.g., HeLa, HEK293) and validate band specificity with siRNA knockdown or competition with the immunizing peptide.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions per sample type. Cytoplasmic and perinuclear staining are expected, but stress granule localization may be observed under stress conditions.
• For immunoprecipitation, confirm efficiency by blotting with an alternative eIF4A1 antibody or by mass spectrometry.
• ELISA performance can be assessed using recombinant protein or peptide-coated plates; titrate the antibody to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers and diagnostic developers focusing on translation initiation and RNA helicase biology, this anti-eIF4A1 polyclonal antibody is available along with full technical support and custom procurement solutions. Our commitment is to streamline your supply chain from concept to clinic.