Anti-EIF3L Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y262

Core Product Specifications and Parameters

Biological Background: EIF3L Function and Localization

• EIF3L (Eukaryotic translation initiation factor 3 subunit L) is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, essential for protein synthesis initiation. The eIF-3 complex stimulates mRNA recruitment to the 43S pre-initiation complex and facilitates AUG recognition. Related references: PMID:17581632, PMID:25849773, PMID:27462815
• The eIF-3 complex is required for disassembly and recycling of post-termination ribosomal complexes, preventing premature joining of the 40S and 60S subunits. Related references: PMID:17581632
• EIF3L specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, cell cycling, differentiation, and apoptosis. Related references: PMID:25849773
• During feline calicivirus (FCV) infection, EIF3L plays a role in ribosomal termination-reinitiation, leading to translation of the viral VP2 protein. Related references: PMID:18056426
• EIF3L localizes to the cytoplasm, consistent with its role in translation.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing generates multiple isoforms.
• Other aliases include EIF3EIP, EIF3S11, HSPC021, HSPC025, MSTP005, and EIF3S6IP, reflecting its interactions within the eIF-3 complex.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1-230 of human EIF3L (NP_057175.1), covering the N-terminal region. This design may detect full-length and potentially some isoforms; validate in your specific experimental context.
• For Western blot (WB), a band at approximately 67kDa is expected. Use appropriate reducing conditions and confirm with relevant positive controls (e.g., HeLa, HEK293 lysates) and loading controls.
• In immunohistochemistry (IHC-P), anticipate cytoplasmic staining. Optimize antigen retrieval and antibody dilution for tissue samples.
• Cross-reactivity is reported with human, mouse, and rat. For other species, verify sequence homology before use.
• This polyclonal antibody may exhibit batch-to-batch variability; consider including consistent positive/negative controls in each experiment.

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CamelBio serves as a comprehensive one-stop platform for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for diagnostic developers. For translation initiation factors such as EIF3L, CamelBio provides rare target raw-material access, supporting your assay development from concept to clinical application. Explore our portfolio to streamline your procurement and accelerate your diagnostic projects.