Anti-EIF3K Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBQ5

Core Product Specifications and Parameters

Biological Background: EIF3K Function and Localization

• EIF3K (also known as eIF-3 p25/p28) is a subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for multiple steps in protein synthesis initiation, including 43S pre-initiation complex assembly, mRNA recruitment, and ribosomal recycling.
  Related references: PMID:17581632, PMID:25849773, PMID:27462815
• The eIF-3 complex also targets a subset of mRNAs involved in cell proliferation, differentiation, and apoptosis, exerting translational activation or repression through RNA stem-loop interactions.
• Localizes to both the nucleus and cytoplasm, suggesting potential extra-ribosomal functions.
• Ubiquitously expressed, with highest levels in brain, testis, and kidney.
• Subject to post-translational acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing yields multiple isoforms, contributing to functional diversity.
• Classified under keywords: Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Initiation factor, Nucleus, Phosphoprotein, Protein biosynthesis, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human EIF3K (amino acids 1–218). It may detect both denatured and native forms in Western blotting; consider using appropriate reducing and heat-denaturing conditions.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer pH 6.0) are recommended, and staining should be validated with positive and negative tissue controls.
• In ELISA, optimize coating buffer pH and antibody concentrations; include relevant controls to assess specificity.
• Cross-reactivity with mouse and rat EIF3K is expected; verify sequence homology and experimental conditions for each species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, supplying diagnostic manufacturers and research institutes with validated antibodies, ancillary reagents, and custom sourcing for rare targets like EIF3K. Leveraging our network, you can access optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and technical consulting to accelerate workflows in protein synthesis research. Contact us to explore how CamelBio can streamline your translational initiation studies with reliable raw materials.