Anti-eIF3e Monoclonal Antibody for WB, IHC-P, ELISA - P60228

Core Product Specifications and Parameters

Biological Background: eIF3e Function and Localization

• Eukaryotic translation initiation factor 3 subunit E (eIF3e), also known as INT6, EIF3S6, or eIF3-p48, is encoded by the EIF3E gene (Gene ID: 3646).
• Component of the eIF-3 complex required for several steps in protein synthesis initiation, including 43S pre-initiation complex formation, mRNA recruitment, ribosomal subunit recycling, and prevention of premature 40S-60S joining. Related references: PMID:17581632, PMID:25849773, PMID:27462815
• The eIF-3 complex specifically targets and regulates translation of mRNAs involved in cell proliferation, differentiation, and apoptosis through RNA stem-loop binding, acting as either activator or repressor. Related references: PMID:25849773
• Required for nonsense-mediated mRNA decay (NMD); may cooperate with UPF2 to divert mRNAs from translation to the NMD pathway. Related references: PMID:17468741
• May interact with MCM7 and EPAS1 and regulate their proteasome-mediated degradation. Related references: PMID:17310990, PMID:17324924
• Localizes to the cytoplasm, nucleus, and PML bodies.
• Ubiquitously expressed; highest levels in appendix, lymph, pancreas, skeletal muscle, spleen, and thymus.
• Subject to acetylation and phosphorylation, as determined by proteomics.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 210–350) lies within a conserved region of eIF3e; considering this, the antibody may show cross-reactivity with mouse and rat, but validation in the relevant sample system is recommended.
• For Western blot, the predicted molecular weight is 52 kDa; consider using a standard dilution series and appropriate loading controls.
• IHC-P may require antigen retrieval optimization; run parallel controls to validate specificity.
• ELISA protocols should incorporate antibody pair screening and specificity assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined, one-stop access to IVD raw materials—from pre-validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. This eIF3e antibody supports research into translation initiation and NMD pathways, offering dependable performance for assay development. Partner with CamelBio for tailored sourcing solutions that accelerate your project from concept to clinic.