Anti-EIF3D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15371

Core Product Specifications and Parameters

Biological Background: EIF3D Function and Localization

• EIF3D is a subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for the initiation of protein synthesis.
• As an mRNA cap-binding component, EIF3D specifically recognizes the 7-methylguanosine cap of a subset of mRNAs, directing the eIF-3 complex to these transcripts. Related references: PMID:27462815
• The eIF-3 complex facilitates the recruitment of initiation factors and assembly of the 43S pre-initiation complex on the 40S ribosomal subunit. Related references: PMID:18599441, PMID:25849773
• EIF3D-containing eIF-3 targets mRNAs involved in cell proliferation, differentiation, and apoptosis, using RNA stem-loop binding for translational activation or repression. Related references: PMID:25849773
• During feline calicivirus (FCV) infection, EIF3D plays a role in the ribosomal termination-reinitiation event leading to VP2 translation. Related references: PMID:18056426
• EIF3D is localized predominantly in the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 64 kDa is expected. Validate protein loading and transfer conditions using appropriate positive and negative controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization steps should be optimized for your specific cell type to ensure antibody accessibility.
• ELISA can be employed for detecting EIF3D in cell or tissue lysates; consider using a standard curve for quantification.
• The antibody cross-reacts with mouse and rat EIF3D, supporting its use in these species; however, always confirm specificity in the relevant model system.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD manufacturers and research laboratories, offering reliable access to raw materials such as this EIF3D polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your translational research in oncology, virology, and cell biology. With end-to-end consulting services, we help streamline your raw-material procurement from discovery to clinical application.