Anti-EIF3C Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99613

Core Product Specifications and Parameters

Biological Background: EIF3C Function and Localization

• EIF3C is a core subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, a large multiprotein assembly essential for the initiation phase of protein synthesis. Related references: PMID:17581632, PMID:25849773, PMID:27462815
• The eIF-3 complex associates with the 40S ribosomal subunit and recruits initiation factors eIF-1, eIF-1A, and the ternary complex eIF-2-GTP-Met-tRNAi to form the 43S pre-initiation complex (43S PIC). Related references: PMID:17581632
• eIF-3 stimulates mRNA recruitment to the 43S PIC and facilitates scanning of the mRNA for the AUG start codon. Related references: PMID:17581632
• Following termination, eIF-3 plays a role in ribosomal subunit recycling and prevents premature 40S-60S joining. Related references: PMID:17581632
• The eIF-3 complex selectively regulates translation of mRNAs involved in cell proliferation, cell cycling, differentiation, and apoptosis via stem-loop binding mechanisms. Related references: PMID:25849773
• EIF3C is predominantly localized in the cytoplasm.
• Post-translational modifications include phosphorylation and acetylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 694-913) of human EIF3C, suggesting recognition of native and denatured forms in WB and IF/ICC applications.
• For Western blotting, use standard SDS-PAGE and transfer conditions; the detected band is expected at ~106 kDa.
• For immunohistochemistry (IHC-P), consider antigen retrieval with citrate buffer (pH 6.0) and optimize primary antibody dilution.
• In IF/ICC, fixation with paraformaldehyde is recommended; a permeabilization step may enhance nuclear/cytoplasmic target detection.
• Validate reactivity in your specific model system, as expression levels may vary between human, mouse, and rat tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive one-stop platform for IVD raw materials. This anti-EIF3C polyclonal antibody supports studies in translation initiation and cell proliferation. We offer a wide range of validated antibody pairs, optimized recombinant antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic and research development.