Anti-eIF3B Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P55884

Core Product Specifications and Parameters

Biological Background: eIF3B Function and Localization

• Eukaryotic translation initiation factor 3 subunit B (eIF3B), also known as PRT1, EIF3S9, eIF3-p110, or eIF3-p116, is a core component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which plays a critical role in protein synthesis by targeting and initiating translation of a subset of mRNAs involved in cell proliferation, cell cycling, differentiation, and apoptosis.
• The eIF-3 complex is required for several steps in translation initiation: it associates with the 40S ribosomal subunit to facilitate recruitment of initiation factors, stimulates mRNA recruitment and scanning for AUG recognition, and is essential for disassembly and recycling of post-termination ribosomal complexes to prevent premature subunit joining. Related references: PMID:17581632 PMID:9388245
• eIF3B is a key RNA-binding subunit that enables the eIF-3 complex to specifically regulate translation of cell proliferation-related mRNAs by using different modes of RNA stem-loop binding, acting as either a translational activator or repressor. Related references: PMID:25849773
• In the context of feline calicivirus (FCV) infection, eIF3B participates in the ribosomal termination-reinitiation event that directs translation of the viral VP2 protein, highlighting its role in host-pathogen interactions. Related references: PMID:18056426
• Subcellularly, eIF3B is predominantly cytoplasmic and can localize to stress granules, membraneless organelles that form under cellular stress conditions to temporarily store mRNAs and regulate translation.
• eIF3B undergoes post-translational modifications including acetylation and phosphorylation, which may modulate its activity and interactions within the translation initiation machinery.
• Structurally, eIF3B contains WD repeat domains and belongs to the WD repeat protein family, which are known for facilitating protein–protein interactions and forming propeller-like structures, consistent with its role in assembling the multi-subunit eIF-3 complex.

Experimental Guidance and Technical Tips

• For Western blot applications, expect a predominant band at approximately 93 kDa corresponding to full-length eIF3B; consider using a loading control and normalizing signals across samples.
• In immunofluorescence (IF/ICC), eIF3B exhibits a cytoplasmic distribution; permeabilization with detergents such as Triton X-100 or saponin is required, and staining may localize to stress granules under conditions of cellular stress.
• The antibody shows cross-reactivity with mouse and rat homologs; however, it is recommended to validate binding specificity and performance in these species using appropriate positive and negative controls.
• For ELISA, titrate the antibody to determine the optimal working concentration; consider a direct or sandwich ELISA format depending on assay requirements and sensitivity.
• Since the immunogen corresponds to a recombinant fragment (amino acids 101–200), the antibody likely recognizes a conserved linear epitope in the N-terminal region; it may be suitable for applications requiring denatured proteins, though native conformation recognition should be empirically verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering one-stop access to validated antibodies, bulk ancillary reagents, and custom sourcing services—from concept to clinic. This anti-eIF3B rabbit monoclonal antibody supports research into translation initiation, cell proliferation, and host-pathogen interactions, making it a valuable tool for diagnostic and life science applications. For manufacturers requiring matched antibody pairs, alternative formats, or rare-target raw materials, CamelBio provides tailored supply solutions to accelerate development and ensure reliable production.