Anti-eIF3B Polyclonal Antibody for WB, IF/ICC, ELISA - P55884

Core Product Specifications and Parameters

Biological Background: eIF3B Function and Localization

• eIF3B is a core subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for several steps in protein synthesis initiation, including 43S pre-initiation complex formation, mRNA recruitment and scanning, as well as disassembly and recycling of post-termination ribosomal complexes. Related references: PMID:17581632 PMID:25849773 PMID:27462815 PMID:9388245
• The eIF-3 complex specifically targets and regulates translation of a subset of mRNAs involved in cell proliferation, differentiation, and apoptosis, employing different RNA stem-loop binding modes to activate or repress translation. Related references: PMID:25849773
• eIF3B is an RNA-binding protein that contributes to the recruitment of initiation factors (eIF-1, -1A, -2:GTP:Met-tRNAi, -5) to the 40S ribosomal subunit, facilitating the formation of the 43S pre-initiation complex. Related references: PMID:17581632 PMID:25849773 PMID:27462815
• Subcellularly, eIF3B is found in the cytoplasm and can localize to stress granules, suggesting roles in translational control under stress conditions.
• In the context of microbial infection, eIF3B plays a role in feline calicivirus (FCV) translation by participating in a ribosomal termination-reinitiation event that leads to VP2 protein synthesis. Related references: PMID:18056426
• Post-translational modifications of eIF3B include acetylation and phosphorylation, which may regulate its function or complex assembly.
• The protein is characterized by the presence of WD repeats, which are involved in protein-protein interactions, and it undergoes alternative splicing, producing isoforms.
• eIF3B is also known as PRT1 homolog, eIF3S9, eIF-3-eta, eIF3 p110, and eIF3 p116. The calculated molecular weight is approximately 92.5 kDa, but it migrates around 93 kDa by SDS-PAGE.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 93 kDa is expected. Consider using whole cell lysates or cytoplasmic extracts from human, mouse, or rat samples, and include appropriate positive and negative controls to confirm specificity.
• In immunofluorescence/immunocytochemistry (IF/ICC), ensure proper cell fixation and permeabilization. Staining is predominantly cytoplasmic; under stress conditions, eIF3B may localize to stress granules, so consider inducing stress for observed granule formation.
• For ELISA applications, the polyclonal nature and recognition of multiple epitopes may enhance sensitivity, but lot-to-lot variability should be controlled by standardization.
• Since this is a rabbit polyclonal antibody raised against human eIF3B, validation on endogenous target in the intended sample system is recommended, especially when switching between species (human, mouse, rat).
• Although not listed in the standard applications, the antibody may be suitable for immunoprecipitation of the eIF-3 complex; however, this must be empirically tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, bulk ancillary reagents, and rare-target sourcing. For translation initiation and protein synthesis studies, we supply this anti-eIF3B polyclonal antibody alongside complementary products such as matched antibody pairs and optimized reagents for your workflow. Let CamelBio bridge your raw material needs from concept to clinic.