Anti-EIF3A Polyclonal Antibody for WB, IF/ICC, ELISA - Q14152

Core Product Specifications and Parameters

Biological Background: EIF3A Function and Localization

• EIF3A is an RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for multiple steps in the initiation of protein synthesis. Related references: PMID:17581632, PMID:25849773
• The eIF-3 complex, containing EIF3A, facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:Met-tRNAi, and eIF-5 to the 40S ribosome to form the 43S pre-initiation complex (43S PIC). Related references: PMID:17581632, PMID:25849773
• EIF3A stimulates mRNA recruitment to the 43S PIC and scanning for AUG recognition, and it is required for disassembly and recycling of post-termination ribosomal complexes, preventing premature 40S-60S subunit joining. Related references: PMID:11169732, PMID:17581632
• This subunit specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, differentiation, and apoptosis, using different modes of RNA stem-loop binding to exert translational activation or repression. Related references: PMID:25849773, PMID:27462815
• During microbial infection, EIF3A is essential for initiation on type-1 viral internal ribosomal entry sites (IRESs), such as those of HCV, PV, EV71, or BEV, and plays a role in the ribosomal termination-reinitiation event for FCV VP2 translation. Related references: PMID:23766293, PMID:24357634, PMID:18056426
• EIF3A localizes to the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation; the protein contains coiled-coil and RNA-binding motifs and participates in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–250 at the N‑terminus of human EIF3A. Consider this when designing complementary detection reagents or epitope mapping studies.
• For Western blot (WB), the predicted molecular weight is ~167 kDa. Use reducing SDS‑PAGE conditions and validate the observed band in your specific cell or tissue lysates.
• For immunocytochemistry/immunofluorescence (IF/ICC), fixation with 4% paraformaldehyde and permeabilization with 0.1–0.3% Triton X‑100 are commonly suitable. Confirm optimal conditions in your cellular model.
• When using ELISA, appropriate coating buffers and detection systems should be optimized. Consider pairing with a validated capture antibody for sandwich ELISA development.

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CamelBio provides diagnostic manufacturers and researchers with streamlined access to high‑quality IVD raw materials for translation initiation studies, including validated antibody pairs, optimized polyclonal antibodies like anti‑EIF3A, and bulk ancillary reagents. Our one‑stop sourcing platform helps you rapidly secure specialty raw materials, from rare targets to established reagents, supporting your workflow from early‑stage research to clinical application.