Anti-EIF2S3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P41091

Core Product Specifications and Parameters

Biological Background: EIF2S3 Function and Localization

• EIF2S3, also known as eIF2γ, is the gamma subunit of eukaryotic translation initiation factor 2 (eIF2), playing a central role in protein synthesis.
• As part of the eIF2 complex, it forms a ternary complex with GTP and initiator tRNA (Met-tRNAi), which binds to the 40S ribosomal subunit to initiate translation (PubMed:31836389). Related references: PMID:31836389
• After mRNA binding and formation of the 43S pre-initiation complex, GTP hydrolysis on eIF2 triggers release of the eIF2-GDP binary complex, allowing 60S subunit joining and 80S initiation complex formation.
• The GDP-bound eIF2 is recycled to GTP-bound form by the guanine nucleotide exchange factor eIF2B, enabling another round of initiation.
• EIF2S3 is localized to the cytoplasm and cytosol, consistent with its role in translation.
• It is expressed in multiple tissues including testis, brain, liver, and muscle.
• Post-translational modifications include acetylation and phosphorylation, and mutations in EIF2S3 are associated with MEHMO syndrome (a disease characterized by intellectual disability, epilepsy, and obesity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-250) of human EIF2S3, which is well-conserved across species, potentially explaining the cross-reactivity with mouse and rat.
• For Western blot, a band around 51 kDa is expected; consider using reducing conditions and appropriate loading controls.
• In IHC-P, antigen retrieval methods may be necessary; validate with known positive control tissues such as testis or brain.
• This antibody can be used in ELISA; consider testing serial dilutions to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials, including validated antibody pairs and recombinant proteins. Our EIF2S3 polyclonal antibody supports research in protein synthesis and translation regulation, and we offer bulk and customized sourcing solutions for your assay development needs.