Anti-EIF2S2 Rabbit Polyclonal Antibody for WB, ELISA - P20042

Core Product Specifications and Parameters

Biological Background: EIF2S2 Function and Localization

• EIF2S2 encodes the beta subunit of eukaryotic translation initiation factor 2 (eIF2), a heterotrimeric complex essential for protein synthesis.
• eIF2 forms a ternary complex with GTP and initiator methionyl-tRNA, which then binds the 40S ribosomal subunit to form the 43S pre-initiation complex. Related references: PMID:31836389
• After mRNA binding, the 43S PIC joins with the 60S subunit to form the 80S initiation complex; GTP hydrolysis on eIF2 triggers release of eIF2-GDP.
• eIF2 recycling requires GDP-to-GTP exchange catalyzed by eIF2B, a key regulatory step in translation initiation.
• Subcellular localization is primarily cytoplasmic and cytosolic.
• The protein features acetylation, phosphorylation, and ubiquitination modifications and harbors a zinc-finger domain, reflecting roles in metal binding and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-175) of human EIF2S2, which includes the zinc-finger domain; consider this when designing experiments targeting full-length versus truncated isoforms.
• For Western blot applications, a band around 38 kDa is expected; confirm specificity using appropriate controls.
• Cross-reactivity with mouse and rat is reported; validate in the relevant sample system and consider potential species-specific variations.
• Optimize antibody dilution and incubation conditions for your specific ELISA or Western blot protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers and research laboratories a streamlined supply chain for high-quality antibodies, including EIF2S2-related reagents for translation initiation studies. From validated antibody pairs to bulk monoclonal/polyclonal antibodies and ancillary reagents, we support your procurement needs from concept to clinic. For rare or custom targets, our team provides expert sourcing and technical consultation.