Anti-eIF2C3 Monoclonal Antibody for WB, IHC-P, ELISA - Q9H9G7

Core Product Specifications and Parameters

Biological Background: eIF2C3/Argonaute-3 Function and Localization

• eIF2C3, also known as Argonaute-3 (Ago3) or Eukaryotic translation initiation factor 2C 3, is a core component of the RNA-induced silencing complex (RISC) involved in post-transcriptional gene silencing.
• It binds to short RNAs such as miRNAs and represses translation of complementary mRNAs. In stem cells, it has been proposed to stabilize siRNAs derived from Alu repeats and facilitate degradation of a subset of coding mRNAs by recruiting the mRNA decapping complex via EDC4.
• eIF2C3 possesses RNA slicer (endonucleolytic) activity but is highly selective, cleaving only RNAs with specific 5′- and 3′-flanking sequences relative to the guide–target complementarity region (PubMed:29040713). Related references: PMID:29040713
• The protein localizes to the cytoplasm and is concentrated in P-bodies, cytoplasmic granules involved in mRNA storage and degradation.
• eIF2C3 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its stability and activity.
• The AGO3 gene is alternatively spliced, producing multiple isoforms that may have distinct functions or expression patterns.
• It is classified under several functional keywords: it is an RNA-binding protein, endonuclease, hydrolase, metal-binding protein, and ribonucleoprotein, underscoring its catalytic and structural roles in RNA silencing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 721-860) of human eIF2C3, which is part of the PIWI domain; this domain is critical for endonuclease activity and guide RNA binding. Researchers should confirm that the epitope is accessible under their experimental conditions.
• For Western blotting (WB), expect a band at approximately 97 kDa. Optimize sample preparation with appropriate lysis and reducing conditions, and include both positive (e.g., HeLa) and negative (e.g., knockout) controls to verify specificity.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) and validate staining by comparing with known eIF2C3 expression patterns or using siRNA-treated tissue sections.
• In ELISA applications, ensure proper coating of the recombinant protein or peptide and test across a range of antibody concentrations to determine optimal sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support, streamlining the development process from concept to clinic. For researchers targeting RNAi pathways and Argonaute proteins, this anti-eIF2C3 antibody is just one example of our extensive catalog—we also supply validated antibody pairs, recombinant proteins, and ancillary reagents for immunoassay development. Our team can assist with sourcing rare-target raw materials and optimizing assay performance, ensuring you have the resources needed for reliable diagnostic manufacturing.