Anti-EIF2B4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UI10

Core Product Specifications and Parameters

Biological Background: EIF2B4 Function and Localization

• EIF2B4 encodes the delta subunit of the heteropentameric eIF2B complex, a guanine nucleotide exchange factor (GEF) essential for translation initiation.
• The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 gamma subunit, recycling eIF2 to its active GTP-bound form to deliver initiator methionyl-tRNA to the ribosome (PubMed:25858979, PubMed:27023709, PubMed:31048492). Related references: PMID:25858979, PMID:27023709, PMID:31048492
• GEF activity is inhibited when eIF2α is phosphorylated, leading to reduced ternary complex formation and global repression of protein synthesis under stress conditions (PubMed:25858979, PubMed:31048492). Related references: PMID:25858979, PMID:31048492
• The protein localizes predominantly to the cytoplasm/cytosol, consistent with its role in cytosolic translation.
• Post-translational modifications include acetylation and phosphorylation, which may modulate complex assembly or activity.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• Mutations in EIF2B4 are associated with leukodystrophy, highlighting its critical role in neural homeostasis.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 58 kDa; validate the band size using lysates from human, mouse, or rat cell lines.
• In immunofluorescence/immunocytochemistry, confirm cytoplasmic localization consistent with the known subcellular distribution of eIF2B.
• This polyclonal antibody can be used in ELISA; consider a suitable coating antigen and detection system for quantitative assays.
• As a polyclonal reagent, it may exhibit lot-to-lot variability; always include appropriate positive and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-EIF2B4 pAb, and bulk ancillary reagents. Whether you are developing assays for translation initiation studies or rare target sourcing, our one-stop supply model supports every stage from concept to clinic.