Anti-EIF2B3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NR50

Core Product Specifications and Parameters

Biological Background: EIF2B3 Function and Localization

• EIF2B3 is the gamma subunit of the heteropentameric eukaryotic translation initiation factor 2B (eIF2B) complex.
• eIF2B functions as a guanine nucleotide exchange factor (GEF) for eIF2, catalyzing the exchange of GDP for GTP on the eIF2 gamma subunit, which is essential for translation initiation. Related references: PMID:25858979, PMID:27023709, PMID:31048492
• The GEF activity of eIF2B is inhibited when the eIF2 alpha subunit is phosphorylated, leading to reduced availability of methionyl-initiator tRNA and global translation repression. Related references: PMID:25858979, PMID:31048492
• Subcellularly, EIF2B3 localizes primarily to the cytoplasm and cytosol.
• EIF2B3 undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing has been reported.
• Mutations in EIF2B3 are associated with leukodystrophy, highlighting its critical role in cell function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-270) of human EIF2B3, which may favor detection of specific isoforms or processed forms.
• For Western blotting, consider the predicted molecular weight of approximately 50 kDa and optimize sample preparation to preserve phosphorylation status if needed.
• Validate antibody specificity in samples from human, mouse, and rat as cross-reactivity is reported; for non-human species, run appropriate controls.
• In immunofluorescence/ICC, expect a primarily cytosolic/cytoplasmic staining pattern; include a nuclear counterstain to confirm localization.
• For ELISA applications, use the recombinant protein immunogen as a positive control to establish standard curves and detection limits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, technical services, and consulting support. For studies on translation initiation and the integrated stress response, this anti-EIF2B3 antibody provides a reliable tool, and CamelBio can further assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and hard-to-find target raw materials to streamline your project from concept to clinic.