Anti-EIF2B2/eIF2Bbeta Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49770

Core Product Specifications and Parameters

Biological Background: EIF2B2 Function and Localization

• EIF2B2 encodes the beta subunit of the eukaryotic translation initiation factor 2B (eIF2B) complex, also known as eIF2Bbeta or eIF-2Bbeta; it is essential for protein synthesis regulation.
• As a component of the eIF2B complex, EIF2B2 catalyzes the exchange of GDP for GTP on the gamma subunit of eIF2, a critical step for translation initiation. Related references: PMID:25858979 | PMID:27023709 | PMID:31048492
• The guanine nucleotide exchange factor (GEF) activity of eIF2B is inhibited when the alpha subunit of eIF2 is phosphorylated, thereby reducing methionine-charged initiator tRNA availability and repressing global translation under stress. Related references: PMID:25858979 | PMID:31048492
• EIF2B2 is primarily localized in the cytoplasm and cytosol, consistent with its role in translation initiation.
• According to UniProt keywords, EIF2B2 is associated with disease variants linked to leukodystrophy, highlighting its clinical relevance in neurodegenerative disorders.
• The protein has a molecular weight of approximately 39 kDa, as determined by sequence analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-120) of human EIF2B2; therefore, epitopes recognized are likely within this domain.
• For Western blot (WB), a specific band at approximately 39 kDa is anticipated; consider using whole-cell lysates from human, mouse, or rat cell lines.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate staining in the expected cytoplasmic/cytosolic compartment, and include both positive and negative control tissues or cells.
• Cross-reactivity with mouse and rat makes this antibody a candidate for translational research in rodent models; however, verify performance in the intended species and application by pilot testing.
• For immunoprecipitation (IP), the polyclonal nature may aid in capturing native eIF2B complexes; optimize buffer conditions to preserve protein interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-EIF2B2 antibody supports research into translation initiation and related disorders like leukodystrophy. Explore our comprehensive supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your IVD development.