Anti-EIF2B1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q14232

Core Product Specifications and Parameters

Biological Background: EIF2B1 Function and Localization

• EIF2B1 encodes the alpha subunit of the heteropentameric eIF2B complex, which functions as a guanine nucleotide exchange factor (GEF) for eukaryotic initiation factor 2 (eIF2).
• The eIF2B complex catalyzes the exchange of GDP for GTP on the gamma subunit of eIF2, and this GEF activity is repressed when eIF2 alpha is phosphorylated, thereby coupling stress signals to global translation control. Related references: PMID:25858979, PMID:27023709, PMID:31048492
• EIF2B1 is predominantly localized to the cytoplasm, specifically within the cytosol.
• Alternative splicing of the EIF2B1 gene produces multiple transcript variants, which may translate into distinct protein isoforms.
• The protein is subject to acetylation, a common post-translational modification that can influence protein function and stability.
• Mutations in EIF2B subunit genes, including EIF2B1, are associated with leukodystrophy, a spectrum of neurodegenerative disorders.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-305) of EIF2B1; expect detection of full-length protein and possibly N-terminal truncation variants.
• In Western blot, the predicted molecular weight is approximately 34 kDa; validate the banding pattern in relevant human or mouse cell lysates considering potential isoform variation.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions, as the cytoplasmic localization may require mild permeabilization protocols.
• As a polyclonal antibody, it may detect multiple epitopes; include appropriate controls (e.g., knockdown lysates) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner offering one-stop access to validated antibodies, bulk reagents, and technical sourcing support for diagnostic development. For EIF2B1 and translation-related targets, we provide optimized polyclonal and monoclonal antibodies, ancillary reagents, and custom sourcing for rare targets to streamline your research and assay development.