Anti-eIF2B epsilon (EIF2B5) Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13144

Core Product Specifications and Parameters

Biological Background: eIF2B epsilon (EIF2B5) Function and Localization

• eIF2B epsilon is a subunit of the eIF2B complex, which functions as a guanine nucleotide exchange factor (GEF) for eIF2, catalyzing GDP to GTP exchange on the eIF2 gamma subunit to enable translation initiation. Related references: PMID:25858979, PMID:27023709, PMID:31048492
• The GEF activity of eIF2B is inhibited upon binding to phosphorylated eIF2-alpha, leading to reduced methionyl-initiator tRNA availability and global translation repression during stress responses. Related references: PMID:25858979, PMID:31048492
• eIF2B epsilon localizes predominantly to the cytosol and cytoplasm, consistent with its role in cytoplasmic translation initiation.
• Post-translational modifications such as acetylation, methylation, and phosphorylation, along with ubiquitination (isopeptide bond), suggest dynamic regulatory control.
• Mutations in EIF2B5 are causative for leukodystrophy with vanishing white matter (VWM), also known as childhood ataxia with central hypomyelination (CACH), underscoring its importance in neurological health.
• The protein belongs to the translation initiation factor family and is part of the eIF2B complex, which is essential for protein biosynthesis and is identified as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen (aa 442-721) corresponds to the C-terminal half of eIF2B epsilon; this region may be well-exposed in the native complex, making the antibody potentially useful for IP and IF/ICC applications.
• In WB, a specific band at approximately 80 kDa should be detected; verify specificity using lysates from human, mouse, or rat cell lines.
• For ELISA, consider using the antibody as a capture or detection reagent in sandwich or indirect formats; appropriate pairing should be validated empirically.
• When performing IP, include appropriate controls such as pre-immune rabbit IgG to assess non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this eIF2B epsilon (EIF2B5) rabbit pAb, and bulk ancillary reagents. From concept to clinic, our supply capabilities support the development of assays targeting translation initiation and leukodystrophy biomarkers. For rare target sourcing and technical consulting, CamelBio bridges your research and product needs.