Anti-eIF2B epsilon (EIF2B5) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13144

Core Product Specifications and Parameters

Biological Background: eIF2B epsilon (EIF2B5) Function and Localization

• EIF2B5 encodes the epsilon subunit of the translation initiation factor eIF2B, a heteropentameric complex that functions as a guanine nucleotide exchange factor (GEF) for eukaryotic initiation factor 2 (eIF2) (PubMed:25858979, PubMed:27023709, PubMed:31048492). Related references: PMID:25858979 PMID:27023709 PMID:31048492
• The eIF2B complex catalyzes the exchange of GDP for GTP on the gamma subunit of eIF2, a critical step for recycling eIF2 to its active GTP-bound form and initiating new rounds of mRNA translation (PubMed:25858979, PubMed:27023709, PubMed:31048492). Related references: PMID:25858979 PMID:27023709 PMID:31048492
• eIF2B GEF activity is tightly regulated by cellular stress; phosphorylation of the alpha subunit of eIF2 converts eIF2 from a substrate to a competitive inhibitor of eIF2B, leading to global repression of protein synthesis (PubMed:25858979, PubMed:31048492). Related references: PMID:25858979 PMID:31048492
• This protein localizes predominantly to the cytoplasm and cytosol, consistent with its role in cytoplasmic translation initiation.
• EIF2B5 is subject to multiple post-translational modifications, including phosphorylation and acetylation, and is implicated in leukodystrophy-related disease variants (e.g., CACH/VWM syndrome), highlighting its importance in maintaining translational control in oligodendrocytes and astrocytes.
• The eIF2B complex is evolutionarily conserved, and structural studies provide insight into its regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human eIF2B5, an N-terminal region that may provide specificity for the epsilon subunit; consider verifying reactivity with recombinant protein or cell lysates.
• In western blotting, a band at approximately 80 kDa is expected; include appropriate positive and negative controls to confirm detection.
• For IHC-P and IF/ICC, optimize antigen retrieval and antibody dilution in the relevant tissue or cell system, as eIF2B5 expression can vary with cell stress and type.
• ELISA assays may be used for quantitative analysis; pair with a suitable detection system and validate with known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with end-to-end IVD raw material supply, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This eIF2B5 monoclonal antibody enables investigation of translation initiation, stress signaling, and leukodystrophy mechanisms, with CamelBio’s dedicated sourcing ensuring access to consistent, high-quality materials. Our team also facilitates rare target raw-material procurement, accelerating your development from concept to clinic.