Anti-EIF2AK1 Rabbit Monoclonal Antibody for WB, ELISA - Q9BQI3

Core Product Specifications and Parameters

Biological Background: EIF2AK1 Function and Localization

• EIF2AK1, also known as heme‑regulated inhibitor (HRI) or heme‑controlled repressor (HCR), is a metabolic‑stress sensing serine/threonine kinase that phosphorylates the α subunit of eukaryotic translation initiation factor 2 (eIF‑2α) on serine 51. This phosphorylation converts eIF‑2α into a global protein synthesis inhibitor, thereby attenuating cap‑dependent translation and initiating the integrated stress response (ISR).
  Related references: PMID:32132706, PMID:32132707, PMID:37327776

• As a heme sensor, EIF2AK1 binds hemin through a cysteine thiolate and histidine nitrogenous coordination with moderate affinity, keeping the kinase inactive under normal conditions. Heme depletion, such as during iron deficiency, releases this inhibition, triggering ISR activation and playing a crucial role in coordinating hemoglobin synthesis in red blood cells.

• Upon mitochondrial dysfunction, the processed form of DELE1 (S‑DELE1) directly binds and activates EIF2AK1, leading to ATF4‑mediated transcriptional reprogramming. This pathway also promotes PRKN‑independent mitophagy through eIF‑2α phosphorylation at damaged mitochondria.
  Related references: PMID:32132706, PMID:32132707, PMID:37327776, PMID:38340717

• The EIF2AK1 gene undergoes alternative splicing, yielding multiple protein isoforms. The protein is post‑translationally modified by phosphorylation and ubiquitin‑like conjugation, as indicated by phosphoproteomic and proteomic studies.

• Subcellular localization is not explicitly described in the current UniProt record; however, all known substrates and functional partners localize to the cytoplasm, consistent with its role in translation regulation.

Experimental Guidance and Technical Tips

• Because the immunogen corresponds to residues 337‑586 of human EIF2AK1, which encompasses a large portion of the kinase domain, this antibody is likely to recognize both full‑length and truncated isoforms where this region is preserved. For Western blot (WB), consider optimizing lysate preparation with phosphatase inhibitors and validate detection in cell lines with confirmed EIF2AK1 expression.
• In ELISA applications, the antibody can be used as a capture or detection reagent; pairing with a different clone or a polyclonal detection antibody may improve assay sensitivity. Validate the assay in the relevant sample matrix.
• Given the [KD Validated] designation, the antibody has been tested in knockdown samples; performing a comparative WB with siRNA‑treated versus untreated cells can further confirm specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one‑stop platform for sourcing IVD raw materials, technical services, and development consulting. This high‑quality recombinant rabbit monoclonal antibody against the stress‑responsive kinase EIF2AK1 supports research and assay development in hematology, mitochondrial biology, and cellular stress fields. Beyond this product, we offer validated antibody pairs, optimized bulk monoclonal/polyclonal antibodies, ancillary reagents, and customized sourcing for rare‑target raw materials, helping you streamline your IVD pipeline from concept to clinic.