Anti-EIF2AK1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BQI3

Core Product Specifications and Parameters

Biological Background: EIF2AK1 Function and Localization

• EIF2AK1 (HRI) is a serine/threonine kinase acting as a metabolic-stress sensor that phosphorylates eIF-2-alpha, inhibiting global translation while promoting ATF4-mediated stress response. Related references: PMID:32132706, PMID:32132707, PMID:37327776
• As a heme sensor, hemin binds to EIF2AK1 and inhibits its activity; heme depletion relieves inhibition, activating the kinase.
• Mitochondrial dysfunction leads to activation via processed DELE1 (S-DELE1), triggering eIF-2-alpha phosphorylation and ATF4-mediated reprogramming. Related references: PMID:32132706, PMID:32132707
• EIF2AK1 also drives mitophagy by phosphorylating eIF-2-alpha, promoting its mitochondrial localization independently of PRKN. Related references: PMID:38340717
• In erythroid cells, EIF2AK1 coordinates heme and globin synthesis, protecting RBCs during iron deficiency.
• EIF2AK1 undergoes alternative splicing and is regulated by phosphorylation and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• The immunogen spans aa 337-586 in the C-terminal region, covering part of the kinase domain; this design is suitable for detecting full-length EIF2AK1 and potential splice variants.
• For Western blotting, a band at approximately 71 kDa is expected; validate specificity using appropriate positive and negative controls (e.g., heme-depleted samples or DELE1-activated lysates).
• In IHC-P, consider antigen retrieval and titrate antibody concentration; known expression in erythroid precursors can serve as a positive control.
• The antibody detects both human and rat EIF2AK1, enabling cross-species validation in translational research models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our EIF2AK1 antibody supports studies on the integrated stress response, mitochondrial dysfunction, and hemolytic disorders. For projects requiring rare-target sourcing or custom development, CamelBio leverages a comprehensive supply network to accelerate your assay and kit development.