Anti-eIF2α Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05198

Core Product Specifications and Parameters

Biological Background: eIF2α Function and Localization

• The protein is eukaryotic translation initiation factor 2 subunit 1 (eIF2α), also known as EIF2S1 or EIF2A. It is a key component of the heterotrimeric eIF2 complex.
• eIF2α forms a ternary complex with GTP and initiator methionyl-tRNA, which then binds the 40S ribosomal subunit to form the 43S pre-initiation complex, a critical early step in cap‑dependent translation. Related references: PMID:16289705, PMID:38340717
• Phosphorylation of eIF2α by stress‑sensing kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK, EIF2AK4/GCN2) is the core event of the integrated stress response (ISR). This modification inhibits global cap‑dependent translation while selectively upregulating stress‑response proteins such as ATF4 and QRICH1. Related references: PMID:19131336, PMID:33384352, PMID:38340717
• Under normal conditions, eIF2α resides predominantly in the cytoplasm and cytosol. It can also localize to stress granules upon cellular stress.
• In response to mitochondrial damage, phosphorylated eIF2α relocalizes to the mitochondrial outer membrane and activates PRKN‑independent mitophagy. Related references: PMID:38340717
• Post‑translational modifications include acetylation and phosphorylation; phosphorylation is the central regulatory switch for translation control under stress.
• The protein possesses RNA‑binding activity and is classified under reference proteome, translation regulation, and initiation factor keywords.

Experimental Guidance and Technical Tips

• The immunogen covers N‑terminal residues 1–260. The antibody may recognize multiple isoforms or homologs that share this domain.
• For Western blotting, a major band around 36 kDa is expected in lysates from human, mouse, and rat cells or tissues.
• Antigen retrieval may be required for immunohistochemistry on paraffin‑embedded sections; optimize dilutions and detection conditions in the relevant sample system.
• ELISA applications should be validated with the appropriate antigen format; consider checkerboard titrations to determine optimal antibody and antigen concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and researchers with streamlined access to high‑quality raw materials, from discovery to clinical application. Our portfolio includes validated polyclonal antibodies like this anti‑eIF2α for studying translation initiation and stress biology, alongside antibody pairs, bulk ancillary reagents, and specialized rare‑target sourcing. Count on us as your integrated partner for all IVD raw‑material needs.