Anti-EIF1AY Rabbit Polyclonal Antibody for WB, ELISA - O14602

Core Product Specifications and Parameters

Biological Background: EIF1AY Function and Localization

• EIF1AY is the Y-chromosomal paralog of EIF1A, also designated eIF-4C, and belongs to the eukaryotic translation initiation factor 1A family.
• It is an essential component of the 43S pre-initiation complex (43S PIC) that binds to the mRNA cap-proximal region and scans the 5' untranslated region to locate the start codon.
• Together with EIF1, EIF1AY facilitates ribosomal scanning, start codon recognition, and assembly of the 48S initiation complex at the initiation codon.
• After start codon recognition, it cooperates with EIF5B to promote joining of the 60S ribosomal subunit and formation of the 80S initiation complex; EIF1AY is released after GTP hydrolysis by EIF5B.
• Its globular domain occupies the ribosomal A site and interacts with EIF5 during scanning to help maintain EIF1 within the open 43S PIC.
• Primarily localized to the cytoplasm, consistent with its role in translation.
• Expressed ubiquitously across human tissues.
• Annotated with isopeptide bond and ubiquitin-like conjugation (Ubl conjugation) sites.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length protein (amino acids 1-144 of human EIF1AY); consider using this antibody in Western blot (predicted band ~16 kDa) and ELISA formats.
• For Western blotting, validate detection in your sample system; the expected molecular weight is approximately 16 kDa.
• Cross-reactivity with mouse and rat is reported; verify specificity via sequence alignment or experimental controls when working with these species.
• EIF1AY is ubiquitously expressed; appropriate positive controls include HeLa or HEK293 lysates; include negative controls to confirm antibody specificity.
• For ELISA applications, test both direct and indirect formats; the antibody may be suitable for capturing recombinant or endogenous EIF1AY, but empirical optimization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams streamlined access to premium IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and auxiliary reagents. This EIF1AY polyclonal antibody supports translation initiation studies and can be sourced alongside complementary ancillary materials from our comprehensive portfolio. For rare or custom targets, our expert team assists with raw-material sourcing and technical consultation to accelerate your assay development.