Anti-eIF1A Monoclonal Antibody for WB, ELISA - P47813

Core Product Specifications and Parameters

Biological Background: eIF1A (EIF1AX) Function and Localization

• eIF1A, also known as eIF-4C, is a translation initiation factor essential for cap-dependent protein synthesis.
• It enhances formation of the cap-proximal complex, facilitates mRNA scanning, and promotes start codon recognition (PubMed:9732867). Related references: PMID:9732867
• After start codon location, it cooperates with EIF5B to orient initiator Met-tRNA and enable 60S subunit joining; its globular domain occupies the A site of the 40S subunit (PubMed:35732735). Related references: PMID:35732735
• During scanning, eIF1A interacts with EIF5 to help maintain EIF1 in the open 43S PIC, though, unlike its yeast ortholog, it does not bind EIF1 directly (PubMed:24319994). Related references: PMID:24319994
• It localizes to the cytoplasm and participates in host-virus interactions, with RNA-binding and tRNA-binding activities.
• As a reference proteome member, it is widely used in studies of translation control and protein biosynthesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 65–144 of human eIF1A, targeting the central region; consider this when designing detection antibodies or epitope mapping.
• Predicted molecular weight is ~16 kDa; for WB, use appropriate gel acrylamide percentage and optimized transfer conditions for low-molecular-weight proteins.
• Cross-reactivity with mouse suggests utility in rodent model studies; always validate performance in your specific sample matrix and experimental system.
• In ELISA applications, consider testing direct vs. sandwich formats and optimize coating antigen concentration and antibody dilutions to maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes as a comprehensive source for IVD raw materials. This anti-eIF1A monoclonal antibody exemplifies our capability to supply validated antibody reagents for translation initiation research. Beyond individual antibodies, we offer antibody pairs, bulk ancillary reagents, and rare target sourcing, helping streamline assay development from concept to clinical application.