Anti-EI24 Rabbit Polyclonal Antibody for WB, ELISA - O14681

Core Product Specifications and Parameters

Biological Background: EI24 Function and Localization

• EI24 (Etoposide-induced protein 2.4 homolog), also known as PIG8 (p53-induced gene 8 protein), is a key mediator of p53-induced apoptosis, functioning as a negative growth regulator.
• The protein is involved in autophagy, regulating the formation of degradative autolysosomes.
• EI24 localizes to multiple membrane compartments, including the nuclear membrane, endoplasmic reticulum membrane, and cytoplasm.
• It is a transmembrane protein with a predicted molecular weight of approximately 39 kDa (UniProt: 38965 Da).
• Post-translational modifications include acetylation and phosphorylation, as annotated in UniProt.
• Alternative splicing produces multiple isoforms, and mutations in EI24 have been associated with disease variants.
• Keywords from UniProt highlight its roles in apoptosis, autophagy, and its presence in membrane-bound organelles.

Experimental Guidance and Technical Tips

• For Western blot, consider using a reducing agent and heating samples appropriately, as EI24 is a transmembrane protein.
• Validate antibody specificity in relevant human, mouse, or rat cell lines, as cross-reactivity is indicated.
• For ELISA, optimize coating antigen and antibody dilution; peptide immunogen may require careful selection of capture/detection conditions.
• Since EI24 is expressed in nuclear and ER membranes, fractionation of cell lysates may enhance detection.
• Note the predicted molecular weight (~39 kDa) and potential for post-translational modifications or isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to validated antibody pairs, optimized polyclonal antibodies like this anti-EI24 pAb, and bulk ancillary reagents for apoptosis and autophagy research. We also support rare target raw-material sourcing to accelerate your IVD development.