Anti-EHMT2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96KQ7

Core Product Specifications and Parameters

Biological Background: EHMT2 Function and Localization

• EHMT2 is a histone methyltransferase that specifically mono- and dimethylates histone H3 at Lys-9 (H3K9me1 and H3K9me2) in euchromatin, establishing a repressive epigenetic mark that recruits HP1 proteins to chromatin. Related references: PMID:11316813, PMID:20084102
• It mediates monomethylation of histone H3 at Lys-56 (H3K56me1) during G1 phase, facilitating the interaction between histone H3 and PCNA to regulate DNA replication. Related references: PMID:22387026
• EHMT2 also weakly methylates histone H3 at Lys-27 (H3K27me). Related references: PMID:11316813
• The enzyme methylates histone H1.4 at Lys-26 (H1.4K26me1 and H1.4K26me2). Related references: PMID:19144645
• Beyond histones, EHMT2 methylates non-histone proteins including p53/TP53 at Lys-373 (dimethylation), CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, and KLF12. It also undergoes automethylation. Related references: PMID:20118233, PMID:18438403
• EHMT2 is localized to the nucleus and associates with chromosomes.
• It is ubiquitously expressed with higher levels in fetal liver, thymus, lymph node, spleen, and peripheral blood leukocytes, and lower levels in bone marrow.
• The protein contains ANK repeats and a SET domain, requires zinc and S-adenosyl-L-methionine for activity, and is subject to acetylation, methylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 2-300) of human EHMT2, which is highly conserved across species; therefore the antibody may recognize the target in human, mouse, and rat samples.
• For Western blot (WB), the predicted molecular weight is ~132 kDa; ensure adequate transfer conditions for high-molecular-weight proteins and include proper controls.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and validate staining specificity using tissue samples with known EHMT2 expression, such as spleen or thymus.
• For ELISA, the antibody can be used as a capture or detection reagent; optimization of coating concentration and blocking conditions is recommended.
• Since EHMT2 is a nuclear chromatin modifier, testing the antibody in chromatin immunoprecipitation (ChIP) applications may be worth considering, though validation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. This anti-EHMT2 rabbit monoclonal antibody supports researchers and diagnostic developers working in epigenetics and transcriptional regulation. From concept to clinic, CamelBio streamlines your procurement process with comprehensive technical support and reliable supply.