Anti-EHMT1 Polyclonal Antibody for WB and ELISA - Q9H9B1

Core Product Specifications and Parameters

Biological Background: EHMT1 Function and Localization

• EHMT1 (also known as GLP, G9a-like protein 1) is a histone-lysine N-methyltransferase that specifically catalyzes the mono-, di- and trimethylation of histone H3 at lysine 9 (H3K9me1/2/3) in euchromatin, a hallmark of transcriptional repression. Related references: PMID:12004135

• Through H3K9 methylation, EHMT1 recruits heterochromatin protein 1 (HP1) to methylated histones, establishing repressive chromatin domains. It also weakly methylates histone H3 at lysine 27 (H3K27me). Related references: PMID:12004135

• EHMT1 is recruited to chromatin by transcription factors such as E2F6, MGA, MAX, and DP1, and contributes to the silencing of MYC- and E2F-responsive genes during the G0 phase of the cell cycle. Related references: PMID:12004135

• Beyond histones, EHMT1 methylates non-histone proteins including p53/TP53 at lysine 373, modulating p53 function. Related references: PMID:20118233

• EHMT1 has a critical role in adipocyte differentiation: upon recruitment by PRDM16, it mediates H3K9me2/3 at promoters of white adipose-selective genes, promoting brown and beige adipocyte development.

• The protein localizes predominantly to the nucleus and associates with chromosomes, consistent with its chromatin-regulatory functions.

• EHMT1 is widely expressed across human tissues and is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.

• Mutations in EHMT1 are associated with Kleefstra syndrome, a neurodevelopmental disorder characterized by intellectual disability, highlighting the protein’s importance in brain development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–260) of human EHMT1; this segment may be conserved among isoforms and species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight of EHMT1 is approximately 141 kDa; use an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions to ensure efficient blotting of high-molecular-weight proteins.
• The antibody is suitable for ELISA; the recombinant immunogen protein can serve as a coating antigen or positive control to confirm assay performance.
• Because EHMT1 is widely expressed, validate specificity using relevant cell or tissue lysates, and include known negative controls when interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw material sourcing, technical services, and development support. This anti-EHMT1 polyclonal antibody, targeting a key epigenetic regulator, exemplifies our portfolio of validated antibodies for chromatin and biomarker research. Beyond individual reagents, we offer comprehensive supply capabilities including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and targeted sourcing for rare and emerging targets to streamline your IVD development pipeline.