Anti-EHHADH Rabbit Polyclonal Antibody for WB and ELISA - Q08426

Core Product Specifications and Parameters

Biological Background: EHHADH Function and Localization

• EHHADH (Enoyl-CoA Hydratase And 3-Hydroxyacyl CoA Dehydrogenase) encodes the peroxisomal bifunctional enzyme (L-bifunctional protein, MFE1), a trifunctional enzyme catalyzing the hydration and dehydrogenation steps of peroxisomal fatty acid beta-oxidation.
• The enzyme acts on long-chain fatty acids, branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA, and long-chain dicarboxylic acids, playing a central role in lipid metabolism. Related references: PMID:15060085
• It possesses 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta3-delta2-enoyl-CoA isomerase activities, enabling it to handle a diverse range of substrates.
• EHHADH localizes to the peroxisome, the organelle where beta-oxidation of very long-chain fatty acids, branched-chain fatty acids, and dicarboxylic acids occurs.
• The protein is highly expressed in liver and kidney, particularly in the terminal segments of the proximal tubule, with lower levels detected in the brain, consistent with its metabolic roles.
• Alternative splicing generates multiple isoforms, and the protein undergoes post-translational modifications including acetylation and phosphorylation.
• Cooperation with HSD17B4 ensures stereospecific hydration and dehydrogenation of trans-2-enoyl-CoA and 3-hydroxyacyl-CoA, with opposite chiral specificity, highlighting its role in coordinating peroxisomal lipid metabolism. Related references: PMID:15060085

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 444-723 near the C-terminus of human EHHADH; therefore, the antibody is likely to recognize epitopes in this conserved region.
• For Western blot analysis, expect a prominent band at approximately 79 kDa in lysates from human, mouse, and rat tissues known to express EHHADH (e.g., liver, kidney).
• The antibody has been validated for ELISA; for other applications, optimization of dilution factors and incubation conditions is recommended on a per-experiment basis.
• As a polyclonal antibody, it may detect multiple isoforms or cleavage products; include appropriate positive and negative controls to confirm specificity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions a streamlined sourcing portal for high-quality IVD raw materials. This anti-EHHADH polyclonal antibody supports studies of peroxisomal lipid metabolism and related disorders. Beyond single reagents, CamelBio offers validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to accelerate your assay development from concept to clinic.