Anti-EHHADH Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q08426

Core Product Specifications and Parameters

Biological Background: EHHADH Function and Localization

• Encoded by the EHHADH gene, the peroxisomal bifunctional enzyme (also known as L-bifunctional protein, MFE1) is a trifunctional enzyme essential for peroxisomal β-oxidation of long-chain and branched-chain fatty acids.
• Possesses 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta3-delta2-enoyl-CoA isomerase activities, catalyzing two of the four reactions in the pathway (By similarity).
• Together with HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and dehydrogenation of 3-hydroxyacyl-CoA with opposite chiral specificity, contributing to dicarboxylic acid degradation (PubMed:15060085).
  Related references:
  PMID:15060085
• Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, showing optimal isomerase activity for 2,5 double bonds (By similarity).
• Localizes exclusively to the peroxisome, reflecting its role in peroxisomal lipid metabolism.
• Predominantly expressed in liver and kidney, with strong expression in terminal segments of the proximal tubule; lower amounts in brain.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.
• Acts as a key regulator of medium-chain dicarboxylic fatty acid levels, influencing overall fatty acid oxidation pathways (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110-284 within the hydratase domain; the antibody may recognize both full-length and isoform proteins.
• For Western blotting, use whole cell lysates from human liver or kidney tissue, or from cell lines with confirmed EHHADH expression; a band around 79 kDa is expected.
• In immunohistochemistry (paraffin), consider heat-induced antigen retrieval and expect a peroxisome-specific punctate staining pattern.
• For immunofluorescence, permeabilization is required to visualize peroxisomal localization; co-staining with peroxisomal markers can confirm specificity.
• Validate antibody performance in your specific sample matrix and optimize dilutions experimentally, as recommended staining patterns can vary with fixation and processing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides end-to-end IVD raw-material solutions, including validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents for peroxisomal metabolism research. As a dedicated sourcing bridge, we support diagnostic manufacturers with rare-target antibody sourcing and scalable supply, accelerating your assay development from bench to patient.