Anti-EHF Rabbit Monoclonal Antibody for WB, ELISA - Q9NZC4

Core Product Specifications and Parameters

Biological Background: EHF Function and Localization

• EHF (ETS homologous factor), also known as ESE-3, is an ETS domain-containing transcription factor encoded by the EHF gene in humans.
• Functions as both a transcriptional activator and repressor, binding to the consensus core sequence GGAA. It modulates epithelial cell differentiation and proliferation, and regulates mitogen-activated protein kinase signaling cascades.
• Involved in the regulation of TNFRSF10B/DR5 expression by binding to Ets-binding sequences on its promoter, linking EHF to apoptosis and cancer-related pathways.
• May act as a tumor suppressor gene or anti-oncogene, contributing to development and carcinogenesis.
• Localizes exclusively in the nucleus, consistent with its role as a transcription factor.
• Exhibits tissue specificity: highly expressed in epithelial-rich tissues such as salivary gland, mammary gland, prostate, and lung; weakly expressed in kidney and colon; absent in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine, and peripheral blood leukocytes.
• Keywords: alternative splicing, DNA-binding, nucleus, transcription regulation; proteomics identification and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 113 amino acids of human EHF. For Western blot, the predicted molecular weight is 35 kDa; note that alternative splicing may produce isoforms of different sizes.
• For WB, consider using nuclear extracts to enrich the target, given its exclusive nuclear localization.
• For ELISA, validate with recombinant full-length protein or peptide to ensure proper coating and detection.
• Confirmation of specificity in relevant human epithelial cell lines (e.g., prostate or mammary gland cells) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal antibodies, and bulk reagents for transcription factor targets like EHF. Our sourcing platform supports research and diagnostic development in epithelial biology, oncology, and transcriptional regulation. Contact us for flexible, one-stop access to rare and specialized raw materials from concept to clinic.