Anti-EHF Polyclonal Antibody for WB, ELISA - Q9NZC4

Core Product Specifications and Parameters

Biological Background: EHF Function and Localization

• EHF (ETS homologous factor), also known as ESE-3, is an ETS domain-containing transcription factor with roles in epithelial cell regulation.
• Acts as both transcriptional activator and repressor, binding to consensus GGAA sequences to regulate gene expression and modulate MAP kinase signaling.
• Involved in regulation of TNFRSF10B/DR5 expression through its promoter, implicating functions in apoptosis and tumor suppression.
• May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
• Expressed exclusively in epithelial tissues: highly expressed in salivary gland, mammary gland, prostate, and lung; weakly in kidney and colon; absent in most non-epithelial tissues.
• Localizes to the nucleus to exert its DNA-binding and transcriptional regulatory functions.
• Alternative splicing produces multiple isoforms, and the protein is subject to post-translational modifications.
• Keywords: Alternative splicing, DNA-binding, Nucleus, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• This antibody was raised against the N-terminal region (aa 1-113) of human EHF. It is recommended for Western blotting and ELISA applications, and may show cross-reactivity with mouse EHF due to sequence conservation.
• For Western blotting, consider using nuclear extracts from epithelial cell lines such as MCF-7 (breast), LNCaP (prostate), or A549 (lung). Validate band specificity using appropriate negative controls and lysates from non-epithelial tissues.
• Optimal antibody dilutions and incubation conditions should be determined empirically for each assay; include known positive and negative samples to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This EHF antibody is suitable for epithelial biology and oncology research, supported by our extensive inventory of transcription factor antibodies and ancillary reagents. Contact us for bulk orders, custom formulations, or comprehensive raw-material sourcing solutions tailored to your assay development needs.