Anti-EGR4 Rabbit Polyclonal Antibody for WB, ELISA - Q05215

Core Product Specifications and Parameters

Biological Background: EGR4 Function and Localization

• Early growth response protein 4 (EGR4), also known as AT133, NGFI-C, or NGFIC, belongs to the EGR family of C2H2-type zinc-finger transcription factors.
• EGR4 functions as a transcriptional regulator by specifically recognizing and binding to the DNA sequence 5'-GCGGGGGCG-3' (GSG).
• It activates the transcription of target genes whose products are required for mitogenesis and differentiation (By similarity).
• The protein is localized predominantly in the nucleus, consistent with its role in transcription regulation.
• EGR4 contains zinc-finger domains that mediate DNA binding and likely contact metal ions, highlighting its classification as a metal-binding transcription factor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 440-589) of human EGR4. This region may contain unique epitopes, but cross-reactivity with other EGR family members should be assessed in relevant sample systems.
• For Western Blot, the expected band is near 62 kDa; nuclear extracts from human, mouse, or rat cell lines can serve as positive controls.
• In ELISA applications, the recombinant immunogen fragment can be utilized as a coating antigen to determine antibody titer and specificity.
• Validate the antibody in your specific experimental context, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical services. This anti-EGR4 antibody complements transcription-related research and assay development. Beyond individual antibody sourcing, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to streamline your workflow from concept to clinic.