Anti-Egr4 Rabbit Polyclonal Antibody for WB, ELISA - Q05215

Core Product Specifications and Parameters

Biological Background: Egr4 Function and Localization

• Egr4, also known as AT133, is a member of the early growth response (EGR) family of zinc-finger transcription factors.
• It functions as a transcriptional regulator by binding to the specific DNA sequence 5'-GCGGGGGCG-3' (GSG), activating target genes involved in mitogenesis and differentiation (By similarity).
• Egr4 contains C2H2-type zinc-finger domains, which facilitate DNA binding and are characteristic of this transcription factor family.
• It is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications and protein interactions may modulate its activity, as suggested by its zinc-binding and DNA-binding properties.
• Egr4 is implicated in cellular processes such as growth factor responses, with alternative names NGFIC (nerve growth factor-induced protein C) indicating its induction by growth signals.
• Keywords associated with Egr4 include DNA-binding, metal-binding, nucleus, transcription, and transcription regulation, highlighting its central role in gene expression control.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the full-length mouse Egr4 (residues 1-367), which may generate antibodies recognizing both native and denatured forms of the protein.
• For Western blotting (WB), consider using appropriate controls and validate the antibody specificity with blocking peptides or knockdown samples, as Egr4 may have closely related family members (e.g., Egr1, Egr2, Egr3).
• When performing ELISA, ensure optimization of coating antigen and antibody dilution to achieve optimal signal-to-noise ratio, as polyclonal antibodies can exhibit batch-to-batch variability.
• Given the predicted molecular weight of approximately 62 kDa (mouse Egr4), confirm band specificity in WB by comparing with known molecular weight markers and possibly using positive control lysates from mouse tissues expressing Egr4.
• Note that the antibody is validated for mouse reactivity; cross-reactivity with other species (e.g., human, rat) may require experimental verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies, antigen proteins, and ancillary reagents. This anti-Egr4 polyclonal antibody supports research into transcriptional control and cellular differentiation pathways. Leverage CamelBio's global sourcing network to accelerate your diagnostic assay development from concept to clinic.