Anti-EGR3 Rabbit Polyclonal Antibody for WB and ELISA - Q06889

Core Product Specifications and Parameters

Biological Background: EGR3 Function and Localization

• EGR3 (Early growth response protein 3), also known as Zinc finger protein pilot, is a transcription factor encoded by the EGR3 gene.
• It is a probable transcription factor that plays a role in muscle spindle development, crucial for proprioception and motor coordination.
• EGR3 belongs to the early growth response (EGR) family of C2H2-type zinc-finger transcriptional regulators, which includes EGR1, EGR2, and EGR4.
• The protein localizes predominantly to the nucleus, consistent with its function in transcriptional regulation.
• EGR3 contains zinc-finger motifs that mediate DNA binding and transcriptional activity, classified under keywords such as DNA-binding, Metal-binding, and Zinc.
• Alternative splicing of EGR3 generates multiple isoforms, as indicated by the keyword "Alternative splicing" and proteomics identification.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning the N-terminal region (amino acids 1–250) of human EGR3. It may recognize both full-length and truncated isoforms; consider assessing isoform specificity in your experimental system.
• For Western blot, the predicted molecular weight is approximately 43 kDa. Additional bands may appear due to post-translational modifications or alternative splicing; validate with appropriate controls.
• The antibody cross-reacts with mouse EGR3, suggesting potential utility in mouse models, but we recommend preliminary validation in your target tissue.
• For ELISA, the immunogen can serve as a positive control to confirm assay performance; optimize coating and detection conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting researchers and manufacturers from concept to clinic. This EGR3 antibody is suitable for studies in transcriptional regulation and neuromuscular development. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to accelerate your diagnostic development.