Anti-EGR2 Monoclonal Antibody for WB, IP, ELISA - P11161

Core Product Specifications and Parameters

Biological Background: EGR2 Function and Localization

• EGR2, also known as Krox-20, is a sequence-specific DNA-binding transcription factor with zinc finger domains. Related references: PMID:17717711
• It plays a critical role in hindbrain segmentation by regulating Hox gene expression, essential for rhombomere specification.
• In the peripheral nervous system, EGR2 governs Schwann cell myelination via regulation of myelin genes like MPZ.
• EGR2 also functions as an E3 SUMO-protein ligase, facilitating SUMO1 conjugation to coregulators NAB1 and NAB2, which modulates its transcriptional activity.
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include acetylation and sumoylation, and it is associated with neuropathies such as Charcot-Marie-Tooth disease.
• Alternative splicing yields multiple isoforms, and it is classified under keywords like acetylation, activator, alternative splicing, disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), which may detect multiple isoforms; consider validating in the relevant sample system.
• For Western blotting, the predicted protein weight is approximately 50kDa; use appropriate molecular weight markers and loading controls.
• This antibody is suitable for IP and ELISA applications; optimize dilutions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials. For EGR2 research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our supply capabilities support projects in neurobiology, transcriptional regulation, and myelination disorders.