Anti-EGR1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P18146

Core Product Specifications and Parameters

Biological Background: EGR1 Function and Localization

• EGR1 functions as a transcriptional regulator, binding to the DNA sequence 5'-GCG(T/G)GGGCG-3' (EGR-site) in target gene promoters. Related references: PMID:20121949
• It binds double-stranded target DNA irrespective of cytosine methylation status, suggesting epigenetic regulatory roles. Related references: PMID:25258363 PMID:25999311
• EGR1 controls expression of genes governing cell survival, proliferation, and apoptosis; it activates tumor suppressors p53 and TGFB1.
• It is required for normal mitosis and hepatocyte proliferation after liver injury.
• EGR1 mediates cellular responses to ischemia/hypoxia by inducing inflammatory cytokines such as IL1B and CXCL2.
• It contributes to circadian clock regulation by modulating the rhythmic expression of core clock genes in the liver and suprachiasmatic nucleus.
• EGR1 protein is abundant in neutrophilic granulocytes.
• Localizes to both nucleus and cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–250) of human EGR1; cross-reactivity with mouse and rat suggests conserved epitopes. Validate species-specific detection in relevant samples.
• For western blot (WB), a band at approximately 58 kDa is expected; use positive controls such as neutrophil or liver lysates.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions, as EGR1 localizes to both nucleus and cytoplasm.
• For ELISA, consider coating with recombinant EGR1 protein and pairing with a suitable detection antibody; titrate antibody concentrations for optimal signal-to-noise ratio.
• Validate the antibody performance in the intended application and sample type before quantitative experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials and technical support, covering every stage from concept to clinic. As a one-stop partner for transcription factor research and diagnostic development, we offer validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. Our global sourcing capabilities can also accommodate custom requests for rare or bulk raw materials.